Ginseng Pectin WGPA Alleviates Exercise-Induced Fatigue by Enhancing Gluconeogenesis

Figure 1

Effect of WGPA on biochemical parameters in serum from exercise-induced fatigue mice. 10- to 11-wk -old male C57BL/6J mice were subject to the established exercise-induced fatigue model using Rotary Fatigue Tester. Experimental and technical details were included in “Materials and Methods.” The exhausted mice were administrated with WGPA prior to 3 h resting. After determination of body weight (a), serum was collected for the assessment of lactic acid (LA) (b), blood glucose (c), blood urea nitrogen (BUN) (d) and malonaldehyde (MDA) (e) level. The activity of superoxide dismutase (SOD) (f), creatine phosphokinase (CK) (g) and lactate dehydrogenase (LDH) (h), as well as the level of insulin (i) and glucagon (k) in serum were evaluated. (j) Ginseng pectin WGPA downregulates insulin secretion from Min6 cells. Min6 cells were incubated in KRBH buffer supplemented with 25 mM glucose and 15 mM sodium lactate. At the onset of incubation, WGPA was added into the medium with indicated concentrations. After 1 h stimulation, the supernatants were collected for insulin assessment. (a)–(i) and (k) Data represent mean ± SD (n = 6) with significance determined by student unpaired t-test. (j) Data represent mean ± SD (n = 3) with significance determined by one-way ANOVA test. ; ; ns, nonsignificant.

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