Mechanism of Electroacupuncture Analgesia on Nicotine Withdrawal-Induced Hyperalgesia in a Rat Model

<div>Electroacupuncture effects on nAChR <i>α</i>7, nAChR<i>α</i>4, nAChR <i>β</i>2, GLRA3, COX2, and Iba1 protein expressions in the Spinal Cords. Western blot analysis of nAChR <i>α</i>7 (a), nAChR <i>α</i>4 (b), nAChR <i>β</i>2 (c), GLRA3 (d), COX2 (e), and Iba1 (f) in the spinal cord of rats in the three groups (CTR: <i>n</i> = 6, NIC + <i>W</i>: <i>n</i> = 6, NIC + <i>W</i> + EA: <i>n</i> = 6). Statistical tests were analyzed using Student’s <i>t</i>-test. 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103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.05,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.29,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.53,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. the CTR group; <span class="nowrap"><svg height="14.9569pt" id="M10" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -11.5275 49.4726 14.9569" width="49.4726pt" xmlns="http://www.w3.org/2000/svg" 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457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,47.444,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> vs. the NIC + <i>W</i> group). CTR—control group, NIC + <i>W</i>—nicotine withdrawal group, NIC + <i>W</i> + EA—nicotine withdrawal with electroacupuncture group.</div>

Evidence-Based Complementary and Alternative Medicine

fig3

Figure 3

Figure 3: Mechanism of Electroacupuncture Analgesia on Nicotine Withdrawal-Induced Hyperalgesia in a Rat Model 