Research Article
Morroniside Protects Human Granulosa Cells against H2O2-Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways
Figure 5
Effect of morroniside on the protein expression and nuclear translocation of Nrf2. (a) The green fluorescence represents Nrf2, and the nucleus is blue DAPI. The white arrows represent Nrf2 in the cytoplasm and the red arrows represent Nrf2 in the nucleus. Scale bars = 50 μm. The protein expression levels of (b) nuclear Nrf2, (c) p-Nrf2, Nrf2 were detected by western blot analysis, and the protein expression levels of p-Nrf2 in nucleus, p-Nrf2/Nrf2 were quantitatively analyzed. (d) GCs were infected with sh-Nrf2, then treated with morroniside and H2O2. SOD and NQO1 expression were detected by western blot analysis. The concentration of morroniside in (d) was 10 μM. Densitometry analysis of the western blots were shown at the right. Data represent mean ± SD, n = 3. versus the H2O2 group, and & versus the morroniside group.
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