Morroniside Protects Human Granulosa Cells against H₂O₂-Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways

Figure 7

Morroniside downregulates the p38 and JNK signaling pathways to inhibit apoptosis in GCs. (a) GCs were incubated with p38 inhibitor SB203580, and then treated with morroniside and H₂O₂. The protein expression levels of Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were detected by western blot analysis. (b–e) Densitometric analyses for western blots in (a). (f) GCs were incubated with JNK inhibitor SP600125, and then treated with morroniside and H₂O₂. The protein expression levels of Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3were detected by western blot analysis. (g–j) Densitometric analyses for western blots in (f). The concentration of morroniside was 10 μM. Data represent mean ± SD, n = 3. versus the H₂O₂ group, and ^& versus the morroniside group, ^# versus the H₂O₂ + p38 inhibitor group or the the H₂O₂ + JNK inhibitor group.

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