Comparison of Pharmacokinetic Profiles of 14 Major Bioactive Components in Normal and Arthritic Model Rats after Oral Administration of Angelicae pubescentis Radix by UPLC-MS/MS

Table 2

Precision and accuracy of 14 compounds in rat plasma (n = 6).

Analytes	Concentration (ng/mL)	Precision (RSD, %)		Accuracy (RE, %)
		Intraday	Interday	Intraday	Interday
Neochlorogenic acid	0.6	3.76	1.10	4.51	3.64
	10	4.00	9.21	4.21	10.86
	252	2.62	0.89	9.18	10.30
	504	8.31	13.52	2.38	10.70
Chlorogenic acid	10	6.72	4.99	0.75	0.05
	10.6	2.37	3.61	−6.49	−5.29
	1060	2.42	2.16	−2.73	−2.28
	3180	9.06	13.81	−14.76	−12.53
Cryptochlorogenic acid	2.5	4.33	0.99	3.58	2.49
	11.7	2.90	7.63	0.08	13.67
	117	2.97	2.92	5.04	3.55
	234	4.77	1.45	1.01	2.67
3,4-diCQA	10.7	9.33	1.40	4.55	6.19
	11.2	8.82	12.70	8.33	−0.36
	56	5.32	3.40	1.23	0.01
	112	0.82	0.17	5.30	4.83
3,5-diCQA	2.5	9.40	1.71	−1.56	−0.09
	12.5	6.15	5.84	5.17	9.80
	312.5	2.51	4.39	−3.79	−6.89
	625	1.06	2.62	0.61	2.14
4,5-diCQA	10.0	13.00	1.92	1.24	2.03
	20.4	3.17	1.42	−0.96	−0.26
	102	5.00	0.96	1.01	0.42
	204	1.20	2.44	1.36	0.03
Columbianetin	3.9	3.87	0.74	7.29	7.98
	39	8.72	4.37	9.64	6.70
	2260	1.24	3.77	3.63	5.96
	4520	0.72	0.94	−0.83	−0.34
Psoralen	3.7	2.18	2.43	0.27	2.72
	5.05	2.18	7.61	−9.26	13.01
	252.5	5.09	1.36	8.52	6.86
	505	2.73	2.61	7.39	9.30
Xanthotoxin	2.2	5.09	1.06	11.21	10.00
	11.2	6.02	5.90	0.14	3.41
	560	1.55	2.10	−1.18	−0.17
	1120	3.91	3.86	1.65	3.68
Bergapten	4.8	2.78	0.57	−0.95	−0.49
	13	8.09	4.08	9.96	5.18
	340	2.62	9.98	−8.05	−14.83
	680	6.81	3.61	12.28	9.14
Columbianetin acetate	7.4	8.11	1.56	−1.1	0.40
	54	7.45	1.88	4.31	3.04
	2160	1.86	4.52	5.87	5.73
	4320	3.47	1.98	4.25	6.62
Isoimperatorin	8.4	3.16	0.48	−3.08	−2.59
	8.9	0.39	1.74	1.87	2.19
	445	6.26	4.47	−13.39	−11.21
	890	5.63	4.77	2.76	4.52
Osthol	1.0	4.27	0.74	−3.96	−3.33
	5.5	7.70	4.21	−1.64	0.94
	2200	8.17	1.09	2.50	1.41
	5500	3.67	1.41	5.72	7.17
Columbianadin	6.4	14.22	2.09	11.09	13.84
	12.2	4.19	6.69	−10.37	1.22
	1220	9.14	1.66	3.43	1.91
	2440	8.07	2.82	4.83	7.40