Molecular Mechanism Investigation on Monomer Kaempferol of the Traditional Medicine Dingqing Tablet in Promoting Apoptosis of Acute Myeloid Leukemia HL-60 Cells

Figure 6

Kaempferol promotes the expression of apoptotic pathway. (a) Protein expressions of Akt, P-Akt, Bcl2, Cleaved Casp3, and Bax. HL-60 cell treatment presented as in Figure 4(a) and the protein isolation and analysis by western blotting. GAPDH expression served as an internal control. (b) TUNEL findings after kaempferol treatment (25 μM, 50 μM, and 100 μM) and ATRA (10 μM). (c) mRNA expression levels of the indicated genes were measured by qRT-PCR. (d) Coimmunoprecipitation assay shows AKT1-kaempferol interactions in HL-60 cells. Data were expressed as mean ± SD (n ≥ 3). , , , based on Student’s t-test (two groups) or one-way ANOVA and Tukey’s tests for more than two groups.

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