[Retracted] Loureirin A Exerts Antikeloid Activity by Antagonizing the TGF-β1/Smad Signalling Pathway

Figure 1

Loureirin A inhibits and TGF-β1 promotes KF proliferation. (a) The chemical structure of LA. (b) KFs were divided into four groups treated with 50, 30, and 10 μg/ml LA or 10% FBS as a control group for 24 h, 48 h, and 72 h. The CellTiter-Blue cell viability assay showed that LA inhibited cell proliferation. (c) KFs were divided into four groups and treated with 40, 20, 10 ng/ml TGF-β1 or 10% FBS as a blank control for 24 h, 48 h, and 72 h. The results showed that TGF-β1 promoted cell proliferation. (d, e) KFs were divided into four groups and treated with LA or TGF-β1 for 48 h. The RT-qPCR results showed the mRNA levels of Col-I, Col-III, and α-SMA. The data were normalized to the control and are presented as the mean ± SEM. , compared with the control group. Each assay was performed in triplicate and repeated using four independent cell samples (n = 4, 12 samples).

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