Research Article

Protective Effects of Alternanthera sessilis Ethanolic Extract against TNF-α or H2O2-Induced Endothelial Activation in Human Aortic Endothelial Cells

Figure 5

Effect of A. sessilis ethanolic extract on TNF-α-induced extracellular H2O2 production. HAECs were pretreated with various concentrations of A. sessilis (25–200 μg/mL) or 2 μM of simvastatin for 24 h followed by stimulation with 20 ng/mL of TNF-α for 1 h. The assay was performed immediately using H2O2 assay kits after the collection of cell culture supernatant. The results are presented as the mean ± SEM from three independent experiments (n = 3). #P < 0.05 as compared to the unstimulated control.