Arbutin Inhibited Heat Stress-Induced Apoptosis and Promoted Proliferation and Migration of Heat-Injured Dermal Fibroblasts and Keratinocytes by Activating PI3K/AKT Signaling Pathway

Figure 7

Arbutin promoted the proliferation and migration of HaCAT and DFL cells after induced by heat stress by activating the PI3K/AKT pathway. (a) HaCAT and DFL cells treated with solvent, 100 μmol/L arbutin (Ar), and arbutin (100 μmol/L) + LY294002 (50 μmol/L) for 48 hours after 43°C-50 min heat stress; statistically compared the value of OD450 in the detection of CCK8 (n = 3). (b) PCNA protein expression in HaCAT and DFL cells of different groups detected by Western blotting. (c) Statistically compared expression of PCNA protein in (b) (n = 3). (d) Transwell chamber for determining the migration of HaCAT and DFL cells in different groups. (e) Statistically compared the number of cells that have migrated in (d) (n = 3). value was calculated by one-way ANOVA. vs. the control group; ^### vs. the solvent group; ^&&& vs. the Ar group.

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