Chondroprotective Effects of Gubitong Recipe via Inhibiting Excessive Mitophagy of Chondrocytes

Figure 4

The localization of vimentin and DAPI was visualized under fluorescence microscopy after immunofluorescence staining. (a) Antivimentin antibody (green). (b) DAPI staining of nuclei (blue). (c) The merge of (a) and (b). Superficial markers of chondrocytes were detected using flow cytometry, with SW1353 cells as negative control. (d) 98% of chondrocytes show positive for type II collagen. (e) SW1353 cells show negative for type II collagen. (f) Type II collagen mRNA in chondrocytes and SW1353 cells was detected by qRT-PCR.

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