Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-<i>β</i>1/Smad Signaling Pathway

<div>LrB suppressed the expression of PAI-1 and the activation of TGF-<i>β</i>1/Smad signaling pathway in MI/R mice. (a) Representative images showing the distribution of PAI-1 in cardiac tissues after immunofluorescence staining. Scar bar = 50 <i>μ</i>m. (b, c) Relative mRNA and protein expression of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in the cardiac tissues. (d) Western blot analysis for TGF-<i>β</i>1R, TGF-<i>β</i>1, p-Smad2/3, and Smad2/3. Relative protein expression of (e) PAI-1, (f) TGF-<i>β</i>1R, (g) TGF-<i>β</i>1, (h) p-Smad2/3, and (i) Smad2/3 in the cardiac tissues. 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456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,39.874,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,46.114,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.078,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.318,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g 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Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-<i>β</i>1/Smad Signaling Pathway 

Evidence-Based Complementary and Alternative Medicine

Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-β1/Smad Signaling Pathway

Figure 4