Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-<i>β</i>1/Smad Signaling Pathway

<div>LrB suppressed angiotensin (Ang) II-induced activation of TGF-<i>β</i>1/Smad signaling pathway in cardiac fibroblasts (CFs) via regulating PAI-1. (a) Representative fluorescent images of fibroblast-specific protein-1 (FSP1) and <i>α</i>-smooth muscle actin (<i>α</i>-SMA) in CFs. Scar bar = 50 <i>μ</i>m. (b) Western blot analysis for PAI-1 in CFs transfected with empty vector or PAI-1 overexpression (OE) vector. (c, d) Relative mRNA and protein expression of PAI-1 in CFs. (e) Western blot analysis for TGF-<i>β</i>1R, TGF-<i>β</i>1, p-Smad2/3, and Smad2/3. Relative protein expression of (f) TGF-<i>β</i>1R, (g) TGF-<i>β</i>1, (h) p-Smad2/3, and (i) Smad2/3 in CFs. Values are indicated as mean ± SD (<i>n</i> = 3). <svg height="14.6307pt" id="M18" style="vertical-align:-3.834001pt" version="1.1" viewbox="-0.0498162 -10.7967 68.0304 14.6307" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M556 236V289H337V504H275V289H56V236H275V-4H337V236H556Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.56,-5.741)"><path d="M556 236V289H337V504H275V289H56V236H275V-4H337V236H556Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,11.12,-5.741)"><path d="M556 236V289H337V504H275V289H56V236H275V-4H337V236H556Z"></path></g><g transform="matrix(.013,0,0,-0.013,17.241,-1.651)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 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602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.318,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.558,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg><i>vs.</i> Ang II group. <svg height="15.672pt" id="M20" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -11.838 71.1269 15.672" width="71.1269pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M724 428H489V396C558 388 570 377 552 332C533 288 517 255 494 220C431 283 362 343 329 370C366 395 388 414 407 430C430 449 449 478 449 518C449 598 382 657 300 657C209 657 133 590 133 499C133 444 161 403 204 363C116 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602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,58.414,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,64.654,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg><i>vs</i>. Ang II + LrB + vector group.</div>

Evidence-Based Complementary and Alternative Medicine

fig5

Figure 5

Figure 5: Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-<i>β</i>1/Smad Signaling Pathway 

Evidence-Based Complementary and Alternative Medicine

Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-β1/Smad Signaling Pathway

Figure 5