Antibiofilm Activity of Azadirachta indica and Catharanthus roseus and Their Synergistic Effects in Combination with Antimicrobial Agents against Fluconazole-Resistant Candida albicans Strains and MRSA

Table 6

Minimum inhibitory concentration of ethanolic extracts and antimicrobial agents against fluconazole-resistant C. albicans strains and MRSA (NCTC12493).

MIC of plant ethanol extracts against test organisms
Test organisms	AI (mg/L)	Test organisms		CR (mg/L)
CA 1	4.0	CA 1		0.1
CA 2	0.1	CA 2		1.0
CA 3	0.5	CA3		0.3
CA 4	4	CA 4		4.0
MRSA	1.0	MRSA		1.0
MIC of antimicrobial agents against test organisms
Test organisms	Fluconazole (μg/mL)	Voriconazole (μg/mL)	Tetracycline (μg/mL)	Ampicillin (μg/mL)	Streptomycin (μg/mL)
CA 1	>64	4.0	NE	NE	NE
CA 2	>64	16.0	NE	NE	NE
CA 3	>64	8.0	NE	NE	>64
CA 4	>64	4.0	NE	NE	NE
MRSA	NE	NE	32.0	16.0	8.0

AI: Azadirachta indica, CR: Catharanthus roseus, CA1: Candida albicans 1, CA2: Candida albicans 2, CA3: Candida albicans 3, CA4: Candida albicans 4, MIC: minimum inhibitory concentration, and NE: not evaluated.