Guanxinping Tablets Inhibit ET-1-Induced Proliferation and Migration of MOVAS by Suppressing Activated PI3K/Akt/NF-κB Signaling Cascade

Figure 6

Effect of GXP on the expression of NF-κB. GXP inhibited the activation of ET-1 on NF-κB signaling pathway. The graphs represent the level of NF-κB for three independent experiments. (a) The protein expression levels of NF-κB. (A) Comparison of NF-κB, GAPDH, and H3 protein expression in nucleus and cytoplasm. (B) The band of NF-κB protein in Western blotting; (b) immunofluorescence staining was performed to determine the expression and distribution of NF-κB, 400x. The expression of NF-κB was red, and nuclei were stained blue with DAPI. The micrographs are representative of three independent experiments; (c) (C, D) the relative expression of NF-κB protein in physiological and pathological groups. (E, F) The ratio of NF-κB fluorescence intensity in physiological and pathological groups. In the physiological group, other groups were compared with the control group, and in the pathological group, other groups were compared with the model group (ET-1 group), , compared with the model group (ET-1 group).