Saireito (114) Increases IC50 and Changes T-Cell Phenotype When Used in Combination with Prednisolone Therapy in Human Peripheral Blood Mononuclear Cells

Figure 4

Dot plot and cell positive rate of CD8⁺ T cells in the PBMCs after PSL and 114 treatments. (a) Dot plot upon gating for CD8⁺ T cells in the PBMCs stimulated by ConA after PSL and 114 treatments (indicated in the square). Gating was conducted using APC-Cy7-A on the vertical axis and FSC-A on the horizontal. The inside of the square frame was CD8⁺ T cells, and the ratio was calculated by the number of CD8-stained cells / lymphocyte count  100. (b) Ratio of CD8⁺ T cells in PBMCs after PSL and 114 treatments. Each treatment was compared to its respective blank using Dunnett’s multiple comparisons test (; ), and the PSL monotherapy group and the PSL + 114 combination therapy groups were compared using the Tukey–Kramer multiple comparison test (mean + SD, n = 6). ConA: concanavalin A, PBMCs: peripheral blood mononuclear cells, PSL: prednisolone, and 114: saireito.

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