Integration of Lipidomics and Transcriptomics Reveals the Efficacy and Mechanism of Qige Decoction on NAFLD

Figure 4

Integrated analysis of lipidomics and transcriptomics. (a-b) The interaction network analysis of DEGs (red rounds) and DELs (blue squares) between the QGL and MOD groups. (a) Interaction network of DEGs and DELs involved in insulin resistance. (b) Interaction network of DEGs and DELs involved in glycerolipid metabolism. The “red” edge represents positive correlation, while the “green” edge represents negative correlation. Edges width represents correlation significance, as determined by PCC. The size of rounds represents the number of DELs related to DEGs. All the LIPID-P-XXXXs in the figure are triglycerides. (c) The correlation coefficient cluster heatmap of DEGs and DELs between the QGL and MOD groups. (d) Histogram of KEGG enrichment for DEGs and DELs between the QGL and MOD groups. (e) The DEGs and DELs involved in related metabolic pathways. The yellow dashed box represents the insulin resistance process and the green dashed box represents the triglyceride biosynthesis process. The rectangle was divided into two equal parts (the left side represents DEGs or DELs in MOD vs CON; the right side represents DEGs or DELs in QGL vs MOD). PPP1R3G: protein phosphatase 1 regulatory subunit 3G; AKT: serine-threonine protein kinase; SREBP1: sterol-regulatoryelement-binding protein 1; GPAT3: glycerol-3-phosphate acyltransferase 3; FAS: fatty acid synthase; ACC: acetyl-CoA carboxylase; FA: fatty acid; G6PC: glucose-6-phosphatase; PGC-1α: alpha peroxisome proliferator-activated receptor gamma coactivator 1-alpha; CREB: cyclic AMP-responsive element-binding protein; CRTC2: CREB-regulated transcription coactivator 2; SIK1: salt-inducible kinase 1; and PPP1R3C: protein phosphatase 1 regulatory subunit 3C.

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