Preclinical Pharmacokinetics, Biodistribution, and Acute Toxicity Evaluation of Caerin 1.9 Peptide in Sprague Dawley Rats

Table 6

Absolute and relative weights of organs of male rats.

Parameters	Single dose				Repeated dose
	Vehicle control	1.0 mg/kg	10.0 mg/kg	100.0 mg/kg	10.0 mg/kg
Body weight (g)	400.42 ± 22.87	380.74 ± 8.63	397.34 ± 21.46	394.58 ± 14.44	342.57 ± 13.86
Brain (g)	2.1834 ± 0.0884	2.163 ± 0.0999	2.181 ± 0.1198	2.1528 ± 0.0816	2.062 ± 0.1162
Heart (g)	1.7608 ± 0.3317	1.485 ± 0.1408	1.728 ± 0.2013	1.6352 ± 0.0401	1.5727 ± 0.082
Liver (g)	12.7004 ± 1.7057	11.4326 ± 0.5122	12.5468 ± 0.8106	12.2242 ± 0.7073	9.9218 ± 0.6297
Spleen (g)	0.8548 ± 0.214	0.7378 ± 0.14	0.8204 ± 0.1012	0.793 ± 0.109	0.741 ± 0.1802
Kidneys (g)	2.988 ± 0.2071	2.8174 ± 0.3469	3.151 ± 0.1131	2.9858 ± 0.1294	2.5043 ± 0.0600
Adrenals (g)	0.059 ± 0.0075	0.0564 ± 0.0078	0.0654 ± 0.0073	0.066 ± 0.0075	0.0612 ± 0.0041
Thymus (g)	0.6074 ± 0.0651	0.545 ± 0.1054	0.6256 ± 0.1022	0.6608 ± 0.082	0.4433 ± 0.0724
Thyroid (g)	0.0478 ± 0.0073	0.0494 ± 0.0051	0.0498 ± 0.0093	0.0482 ± 0.0075	0.0358 ± 0.0092
Testis (g)	3.5324 ± 0.3453	3.328 ± 0.2025	3.2666 ± 0.0813	3.4182 ± 0.3029	3.3857 ± 0.1596
Epididymides (g)	0.9294 ± 0.1662	0.9464 ± 0.0677	0.9564 ± 0.0348	0.9956 ± 0.0459	1.0408 ± 0.0584
Organ-to-body weight ratio (%)
Brain	0.5462 ± 0.0266	0.5684 ± 0.0246	0.5502 ± 0.0414	0.5462 ± 0.0313	0.6027 ± 0.0396
Heart	0.44 ± 0.0831	0.3896 ± 0.0287	0.4362 ± 0.0588	0.4148 ± 0.0148	0.4593 ± 0.0262
Liver	3.161 ± 0.2538	3.0038 ± 0.1462	3.1566 ± 0.0559	3.0962 ± 0.0771	2.8948 ± 0.0939
Spleen	0.212 ± 0.0439	0.1934 ± 0.033	0.2074 ± 0.033	0.201 ± 0.0269	0.2148 ± 0.0437
Kidneys	0.7466 ± 0.0439	0.7386 ± 0.0732	0.794 ± 0.0359	0.757 ± 0.0276	0.7318 ± 0.0218
Adrenals	0.0148 ± 0.0011	0.0148 ± 0.0016	0.0162 ± 0.0018	0.0166 ± 0.0023	0.0178 ± 0.0013
Thymus	0.1526 ± 0.0221	0.1432 ± 0.0257	0.158 ± 0.0301	0.1674 ± 0.0172	0.1292 ± 0.018
Thyroid	0.012 ± 0.0014	0.013 ± 0.0016	0.0126 ± 0.0027	0.012 ± 0.002	0.0105 ± 0.0021
Testis	0.8814 ± 0.0541	0.8742 ± 0.051	0.824 ± 0.0519	0.8678 ± 0.0894	0.9903 ± 0.0744
Epididymides	0.231 ± 0.0319	0.2488 ± 0.0201	0.2412 ± 0.015	0.253 ± 0.0201	0.3047 ± 0.0274
Organ-to-brain weight ratio (g/g)
Heart	80.392 ± 13.13	68.668 ± 5.823	79.07 ± 5.614	76.094 ± 4.612	76.512 ± 6.581
Liver	581.294 ± 71.985	530.106 ± 45.202	576.69 ± 48.769	568.862 ± 45.911	482.405 ± 39.55
Spleen	38.948 ± 8.538	34.02 ± 5.771	37.666 ± 4.57	36.82 ± 4.84	35.907 ± 8.243
Kidneys	136.806 ± 6.963	130.33 ± 15.192	144.666 ± 5.6	138.964 ± 10.232	121.752 ± 6.906
Adrenals	2.704 ± 0.368	2.606 ± 0.333	2.994 ± 0.233	3.066 ± 0.335	2.978 ± 0.32
Thymus	27.848 ± 3.183	25.276 ± 5.165	28.58 ± 3.285	30.734 ± 4.031	21.58 ± 3.924
Thyroid	2.192 ± 0.351	2.292 ± 0.316	2.286 ± 0.415	2.238 ± 0.322	1.737 ± 0.425
Testis	161.986 ± 17.165	153.876 ± 6.445	150.056 ± 7.131	158.602 ± 9.431	164.705 ± 13.717
Epididymides	42.52 ± 7.181	43.792 ± 3.12	43.986 ± 3.405	46.262 ± 1.845	50.623 ± 4.213

Values are expressed as mean ± SD.