Network Pharmacology Analysis and Experimental Verification on Antiangiogenesis Mechanism of Hedyotis diffusa Willd in Liver Cancer

Figure 6

In vivo validation of multipathway effect of HDW. (a) The levels of serum inflammatory factors IL-6, IL-1β, IL-17, and TNF-α were measured in the orthotopic liver cancer mouse models to four different groups. (b) The effect of HDW on the expression of p-Akt1/Akt1, p-mTOR/mTOR, and p-STAT3/STAT3. The tumor lysates were harvested, and phosphorylation of indicated proteins was detected by western blotting. GAPDH was used as the invariant control. (c) Immunohistochemical staining examination of HIF-1α in tumor sections from the vehicle or HDW-treated groups. All panels are of the 200x magnification. Scale bar = 50 μm ( and vs. vehicle).

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