Study on the Mechanism of Qing-Fei-Shen-Shi Decoction on Asthma Based on Integrated 16S rRNA Sequencing and Untargeted Metabolomics

Figure 2

QFSS treatment ameliorated asthma in mice. (a–c) QFSS treatment decreased the W/D ratio of lung tissue (a), total protein concentration (b), and total cell count (c), in BALF in asthmatic model mice (d). The numbers of eosinophils in BALF were observed by Wright’s-Giemsa staining. Mice which received QFSS showed lower eosinophils count in BALF than with mice in the model group. (e) Serum IgE level was tested by ELISA. QFSS treatment reduced the IgE level in mice with asthma. (f) Cytokine levels of IL-4, IL-5, IL-13, and IFN-γ in BALF were investigated using ELISA. QFSS treatment decreased the levels of IL-4, IL-5, and IL-13, and increased the IFN-γ level in BALF in mice with asthma. Control, model, DXM, LD-QFSS, MD-QFSS, and HD-QFSS groups (n = 10 per group). ^## compared with the control group; compared with the model group; compared with the model group.

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