Xueliankoufuye Suppresses Microglial Activation with Inflammatory Pain by Blocking NF-κB Signaling Pathway

Figure 4

Effect of XL on LPS‐induced the NF-κB signaling pathway in BV-2-microglia and spinal cord. (a) XL did not affect cell viability in BV-2 microglia within LPS stimulation. Cell viability was measured using CCK-8 assay, as indicated in Materials and Methods. (b) At 24 h after LPS treatment, XL inhibited LPS‐induced phosphorylation of the NF-κB p65 in BV2 microglia. (c) Orally administration of XL inhibits p65 phosphorylation of the spinal cord in CFA-induced inflammatory joint pain. Data are expressed as means ± SEM (n = 3 in each group). Statistical significance was determined by one-way ANOVA followed by post hoc Tukey analyses where , ^#, ^### vs. control group, vs. LPS group, vs. Joint pain group.

(a)

(b)

(c)