Xueliankoufuye Suppresses Microglial Activation with Inflammatory Pain by Blocking NF-κB Signaling Pathway

Figure 5

Effect of XL on LPS‐induced secretion of proinflammatory cytokines in BV-2-microglia. XL inhibited LPS-induced secretion of IL-6 (a) with IC₅₀ of 20.15 μg/mL (b) and TNF-α (c) with IC50 of 112 μg/mL (d) in BV2 microglial cells. The secretion of IL-6 and TNF-α were quantified using ELISA kits, as described in Materials and Methods. Data are expressed as means ± SEM (n = 3 in each group). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis where ^### vs. control group, vs. LPS group.

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