Evidence-Based Complementary and Alternative Medicine

Research Article

Linggui Qihua Decoction Inhibits Atrial Fibrosis by Regulating TGF-β1/Smad2/3 Signal Pathway

Table 1

The gene primer sequence.
Primer namePrimer sequence (5′ to 3′)Product size (bp)
AT1 receptor upstream primerCAA​CTG​CCT​GAA​CCC​TCT​GT129
AT1 receptor downstream primerAGC​GTG​CTC​ATT​TTC​GTA​GAC
TGF-β1 upstream primerGAG​AGC​CCT​GGA​TAC​CAA​CTA​CTG​C93
TGF-β1 downstream primerCAA​CCC​AGG​TCC​TTC​CTA​AAG​TCA​A
Smad2 upstream primerTTT​GCC​GAG​TGC​CTA​AGT​GAT​A211
Smad2 downstream primerTTC​TTA​TGG​TGC​ACA​TTC​GAG​TC
Smad3 upstream primerGCT​GTC​TAC​CAG​TTG​ACT​CGC​AT135
Smad3 downstream primerGGG​TGC​TGG​TCA​CTG​TCT​GTC​T
MMP9 upstream primerGGA​ACT​CAC​ACA​ACG​TCT​TTC​ACT​A236
MMP9 downstream primerCAG​GAG​GTC​ATA​GGT​CAC​GTA​GG
TIMP-1 upstream primerTTC​CTG​GTT​CCC​TGG​CAT​AAT295
TIMP-1 downstream primerATC​GCT​CTG​GTA​GCC​CTT​CTC
AngIIupstream primerCAC​CCC​TTT​CAT​CTC​CTC​TAC​TA261
AngIIdownstream primerTCT​TGC​CTC​ACT​CAG​CAT​CTT
GAPDH upstream primerTGG​AGT​CTA​CTG​GCG​TCT​T138
GAPDH downstream primerTGT​CAT​ATT​TCT​CGT​GGT​TCA