Research Article

Shoseiryuto Promotes the Formation of a Tight-Junction Barrier in Cultured Human Bronchial Epithelial Cells

Figure 2

Measurement of TEER in a transwell. A transwell is partitioned into apical and basal compartments by an inserted basket (insert). Bronchial epithelial cells seeded on the upper surface of the porous insert membrane (0.4 μm pore size) were cultured in the culture medium. An EVOM3 epithelial voltage resistance meter with a STX2-plus “chopstick” electrodes was used to measure TEER values.