Qinggan Huoxue Recipe Protects against Experimental Alcoholic Liver Fibrosis through CXCL16 Inhibition

Figure 5

QGHXR reduced CXCL16 levels after ethanol plus CCl₄ administration in mice. C57BL/6J mice were either fed a Lieber–DeCarli liquid diet containing 4% ethanol or pair-fed a control diet, and mice were intraperitoneally injected with CCl₄ (0.1 mL/kg) for 8 weeks. At week 5, mice were administered with 100, 200, and 400 mg/kg QGHXR by gavage daily for 4 weeks. (a) The level of CXCL16 in serum were determined by an ELISA assay. (b) The levels of CXCL16 and CXCR6 mRNA in the liver were determined by real-time PCR. (c) The levels of CXCL16 and CXCR6 protein expression in the liver were determined by western blot. The arbitrary units were analyzed by ImageJ software. The values represent means ± SEM (n = 8). , vs. ethanol + CCl₄.

(a)

(b)

(c)