Synergistic Anti-Inflammatory Effects of Ethanol Extracts from Chrysanthemum zawadskii Flower and Cudrania tricuspidata Fruit Occur via Inhibition of the NF-κB Signaling Pathway

Figure 3

Anti-inflammatory signaling effects of ethanol extracts from Chrysanthemum zawadskii flowers. RAW 264.7 cells were treated with the indicated amounts of ethanol extract with or without 0.1 μg/ml of LPS for 30 min. (a) The nuclear translocation of NF-κB subunit was assessed using immunofluorescence. (b) Nuclei were counterstained with DAPI, and positive staining for was quantified and graphed. Scale bar, 10 μm.

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