Synergistic Anti-Inflammatory Effects of Ethanol Extracts from Chrysanthemum zawadskii Flower and Cudrania tricuspidata Fruit Occur via Inhibition of the NF-κB Signaling Pathway

Figure 6

Synergistic anti-inflammatory signaling effect of extracts from Chrysanthemum zawadskii flower and Cudrania tricuspidata fruit. RAW 264.7 cells were treated with 0.1 μg/ml of CT fruit extract and 5 μg/ml of CZ flower extracts alone or cotreated with both extracts. Cells were stimulated with 0.1 μg/ml of LPS for 30 min (a, b) or the indicate time (c). (a) The nuclear translocation of NF-κB subunit was assessed using immunofluorescence. (b) Nuclei were counterstained with DAPI, and positive staining for was quantified and graphed. Scale bar, 10 μm. (c) The expression levels of protein were analyzed by Western blot analysis using anti-IκBα antibodies. β-Actin was detected as a control. The expression of IκBα was quantified and presented graphically. vs. control. ^## vs. LPS treatment.

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