Research Article
Effects of Ninjin’yoeito on Human CYP3A and Mouse CYP3A Activity
Table 2
LC-MS/MS conditions for CYP3A assay HPLC conditions.
| | Instrument | LC-30AD | | Analytical column | CAPCELL PAK C18 MGIII, 5 µm, 2.0 mm I.D. × 50 mm | | Column oven temperature | 40°C (set value) | | Mobile phase | A; 0.1 vol% formic acid | | B; acetonitrile | | Gradient program | Time (min) | 0 | 1 | 1.01 | 2.5 | 2.51 | 3.5 | 3.51 | 6 | | %B | 25 | 25 | 50 | 50 | 90 | 90 | 25 | 25 | | Flow rate | 0.4 mL/min | | Injection volume | 10 µL | | Autosampler temperature | 4°C (set value) | | Injector rinse solution | Acetonitrile/2-propanol/distilled water (2 : 2 : 1, v/v/v) | | MS/MS condition | | Instrument | QTRAP6500 | | Ionization mode | ESI | | Polarity | Positive | | Resolution (Q1/Q3) | Unit/unit | | Duration | 6 min | | Scan type | Multiple reaction monitoring | | Detection conditions | | Analyte | Q1 (Da) | Q3 (Da) | Dwell (msec) | DP (V) | EP (V) | CE (V) | CXP (V) | | 6β-hydroxytestosterone | 305.2 | 269 | 300 | 111 | 10 | 21 | 18 | | 6β-hydraoxytestosterone-d7 | 312.21 | 276.2 | 300 | 71 | 10 | 21 | 18 | | Curtain gas | 40 psi (Nitrogen) | | Ion source gas 1 | 80 psi (air) | | Ion source gas 2 | 80 psi (air) | | Spray voltage | 5000 V | | Heater temperature | 600°C (set value) | | Collision gas | Level 12 |
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