Effects of Ninjin’yoeito on Human CYP3A and Mouse CYP3A Activity

Table 3

HPLC conditions for plasma TRZ analysis.

Instrument	Nexera-i LC-2040C 3D
Analytical column	Inertsil ODS-3 (4.6 mm I.D. × 250 mm, 5 μm, GL sciences)
Guard column	Inertsil ODS-3 (4.6 mm I.D. × 33 mm, 5 μm, GL sciences)
Column oven temperature	40°C
Mobile phase	Distilled water/acetonitrile/methanol (60 : 38 : 2, v/v/v)
Flow rate	1.0 mL/min
Injection volume	20 µL
Detector	PDA (wavelength: 222 nm)