In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line

Table 1

IC₅₀ value in the HepG2 cell line treated with different concentrations of W. somnifera leaf methanolic extract.

Leaf methanolic extracts of W. somnifera on HepG2 cell lines
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	1.143 ± 0.0005	100	0
20	0.9365 ± 0022	81.94 ± 0.046	18.06 ± 0.025
50	0.3915 ± 0.0004	34.25 ± 0.015	65.75 ± 0.028
100	0.2928 ± 0.0001	25.62 ± 0.011	74.38 ± 0.025
200	0.1837 ± 0.0002	16.07 ± 0.017	83.93 ± 0.015
500	0.1149 ± 0.0001	10.05 ± 0.02	89.95 ± 0.068
1000	0.1165 ± 0.0001	10.19 ± 0.015	89.81 ± 0.090
1500	0.1171 ± 0.0001	10.25 ± 0.011	89.75 ± 0.165
2000	0.1164 ± 0.0001	10.19 ± 0.015	89.81 ± 0.035
Log IC50 value	1.634 ± 0.0005
IC50 value	43.06 ± 0.615 μg/mL

IC₅₀ value in bold indicates significant at p ≤ 0.05.