In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line

Table 2

IC₅₀ value in the HepG2 cell line treated with different concentrations of W. somnifera stem methanolic extract.

Effect of stem methanolic extract of W. somnifera in HepG2 cell line
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	1.143 ± 0.0015	100	0
20	0.984 ± 0.0017	86.09 ± 0.020	13.91 ± 0.030
50	0.4139 ± 0.0001	36.21 ± 0.011	63.79 ± 0.025
100	0.2533 ± 0.0001	22.16 ± 0.017	77.84 ± 0.015
200	0.2269 ± 0.0001	19.85 ± 0.015	80.15 ± 0.020
500	0.1144 ± 0.0005	10.01 ± 0.025	89.99 ± 0.046
1000	0.1148 ± 0.0002	10.04 ± 0.025	89.96 ± 0.020
1500	0.1147 ± 0.0004	10.04 ± 0 0.025	89.96 ± 0.026
2000	0.1135 ± 0.0001	9.93 ± 0.051	90.07 ± 0.045
Log IC50 value	1.659 ± 0.0015
IC50 value	45.60 ± 0.3 μg/mL

IC₅₀ value in bold indicates significance at p ≤ 0.05.