In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line

Table 3

IC₅₀ value in the HepG2 cell line treated with different concentrations of W. somnifera root methanolic extract.

Effect of root methanolic extract of W. somnifera in HepG2 cell line
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	1.143 ± 0.0005	100	0
20	1.0972 ± 0.0003	95.99 ± 0.025	4.01 ± 0.068
50	0.9995 ± 0.0009	87.44 ± 0.015	12.56 ± 0.050
100	0.9203 ± 0.0114	80.52 ± 0.020	19.48 ± 0.040
200	0.8236 ± 0.0002	72.06 ± 0.017	27.94 ± 0.023
500	0.3622 ± 0.0004	31.69 ± 0.017	68.31 ± 0.015
1000	0.1631 ± 0.0006	14.27 ± 0.025	85.73 ± 0.011
1500	0.1175 ± 0.0002	10.28 ± 0.035	89.72 ± 0.015
2000	0.1122 ± 0.0002	9.82 ± 0.125	90.18 ± 0.025
Log IC50 value	2.497 ± 0.0015
IC50 value	314.4 ± 0.795 μg/mL

IC₅₀ value in bold indicates not significant at p ≤ 0.05.