Hispidin Inhibits Ferroptosis Induced by High Glucose via the miR-15b-5p/GLS2 Axis in Pancreatic Beta Cells

Figure 3

Hispidin treatment led to the upregulation of miR-15b-5p in PBCs. (a) PBCs treated with hispidin (40 μM) or DMSO for 24 h; the expression of miRNAs was assayed using a microarray technique. (b) PBCs treated with hispidin (40 μM) for 24 h; the expression of miR-15b-5p was analyzed by RT-PCR. PBCs treated with DMSO were used as the control. (c) PBCs transfected with the indicated miRNA mimic/inhibitor for 24 h; the expression of miR-802 was and analyzed by RT-PCR. (d)–(i) Under the HG condition, PBCs transfected with the indicated miRNA mimic/inhibitor for 24 h following which the cells were treated with hispidin (40 μM) for an additional 24 h. Cells cultured under normal conditions were used as the control. (d) Cell viability measured. (e) Cell death measured. (f) ROS levels measured. (g) Fe²⁺ levels measured. (h) MDA levels measured. (i) GSH levels measured. Data are representative of at least three independent experiments ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)