Hispidin Inhibits Ferroptosis Induced by High Glucose via the miR-15b-5p/GLS2 Axis in Pancreatic Beta Cells

Figure 5

Overexpression of GLS2 abrogates the protective effects of hispidin against ferroptosis induced by HG levels in PBCs. (a) PBCs transfected with an empty vector (EV) or GLS2-overexpressing vector (GLS2 OV) for 24 h; protein levels of GLS2 were measured by Western blot. (b)–(g) Under the HG condition, PBCs were transfected with EV or GLS2 OV for 24 h following which the cells were treated with hispidin for an additional 24 h. Cells cultured under normal conditions were used as the control. (b)Cell viability measured. (c)Cell death measured. (d) ROS levels measured. (e) Fe²⁺ levels measured. (f) MDA levels measured. (g) GSH levels measured. Data are representative of at least three independent experiments ().

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