Biofilm-Inhibitory Activity of Wild Mushroom Extracts against Pathogenic Bacteria

Table 2

Primers used to amplify specific genes of the five mushroom species.

Mushrooms	Primers (forward and reverse)	Required genes
Auricularia auricula-judae	ITS1-F (5′-CTT GGT CAT TTA GAG GAA GTA A-3′)	RPB2
	ITS5 (5′-GGA AGT AAA AGT CGT AAC AAGG-3′)
Microporus xanthopus	ITS1-F (5′-CTT GGT CAT TTA GAG GAA GTA A-3′)	ITS nrDNA
	ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′)
Termitomyces umkowaani	ITS1-F (5′-CTT GGT CAT TTA GAG GAA GTA A-3′)	LSU rDNA
	ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′)
Trametes elegans	ITS1-F (5′-CTT GGT CAT TTA GAG GAA GTA A-3′)	TEF1α
	ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′)
Trametes versicolor	ITS1-F (5′-CTT GGT CAT TTA GAG GAA GTA A-3′)	TEF1α
	ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′)

Note. RPB2: RNA polymerase II largest subunit gene; TEF1α: translation elongation factor 1-alpha; LSU rRNA: large subunit ribosomal RNA; ITS nrDNA: internal transcribed spacer nuclear ribosomal DNA.