The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance

Figure 1

Taql, electrophoresis of the GyrA gene after adding the TaqI enzyme. Lane 1, electrophoresis of the GyrA gene prior to adding the enzyme with 441 bp bandwidth; lanes 2, 3, and 4, KpI group; lane 5, KpIII group; lanes 6, 7, and 8, KpII group; lane 9, 50 bp DNA ladder. HaeIII, electrophoresis of the GyrA gene after adding the HaeIII enzyme. Lane 1, 50 bp DNA ladder; lanes 2, 3, and 4, KpI group; lane 5, KpIII group; lanes 6, 7, and 8, KpII group. parC, parC gene electrophoresis to confirm the KpII group with 232 bp bandwidth. Lanes 1, 2, and 3, parC positive isolates with 232 bp bandwidth; lane 4, 100 bp DNA ladder. In KpI strains, the Taql enzyme created four bonds (including 197 bp, 142 bp, 93 bp, and 9 bp) and the HaeIII enzyme created four bonds (including 175 bp, 129 bp, 92 bp, and 45 bp). In KpII strains, the Taql enzyme created three bonds (including 197 bp, 151 bp, and 93 bp) and the HaeIII enzyme created four bonds (including 175 bp, 129 bp, 92 bp, and 45 bp). In KpIII strains, the Taql enzyme created four bonds (including 197 bp, 142 bp, 93 bp, and 9 bp) and the HaeIII enzyme created three bonds (including 175 bp, 174 bp, and 92 bp).