Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours

Table 2

PCR primers’ sequences.

Gene symbol	Primer	Sequence
qRT-PCR
MUPCDH	Forward	CTTCTACGCAGAGGTTGAGG
	Reverse	GGGCTCCTGTTCGGAAAC
ACTB	Forward	AGAGCTACGAGCTGCCTGAC
	Reverse	AAGGTAGTTTCGTGGATGCC
Pyrosequencing
MUPCDH	Forward	TTGGTAGTGGGTTGGATTAG
	Reverse	Biot-AAACCCAAAACCCCATCTTA
	Sequencing	GTGGGTTGGATTAGT
Chip assay
MUPCDH	Forward	CGCTCT CCAGTC CCTTCT G
	Reverse	GACTGA GTGCAG GGGTCA AC
ACTB	Forward	ACGCCTCCGACCAGTGTT
	Reverse	GCCCAGATTGGGGACAAA
HBB	Forward	GCAATAGATGGCTCTGCCCT
	Reverse	GACAGGTACGGCTGTCATCA