Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis

Figure 4

FGF21 antibody administration inhibited experimental colitis-induced eWAT lipolysis. C57BL/6J mice were treated with 2.5% dextran sodium sulfate (DSS) for 7 days, as described in the Materials and Methods. The mice in the WT DSS group were injected with FGF21 antibody (abFGF21) at 5 mg/kg body weight every other day starting from day 3 after colitis was induced. (a) Serum FGF21 protein levels. (b) Serum glycerol. (c) Serum FFA. (d) Immunoblot analysis of eWAT phosphor-HSL, HSL, PLIN, and ATGL protein levels. (e) The quantification of the immunoblot bands.

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