Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats

Figure 4

Effects of EP on Nrf2 and HSP27. (a) Immunohistochemical staining of Nrf2 in the rat livers (×400). ; “→” indicates the positions of Nrf2. (b) Immunohistochemical staining of HSP27 in the rat livers (×400). . (c) The immunofluorescence double staining for Nrf2/CK19. CK19 was stained with green color, Nrf2 was stained with red color, nucleus was stained by DAPI with blue color, and the coexpression of CK19 with Nrf2 within the same cell showed yellow color (merged). ; “→” indicates the positions of CK19/Nrf2/DAPI/merged. (d) Analysis of HSP27 and Nrf2 mRNA expression. The Nrf2 protein level detected by ELISA. (: vs. sham group, , ; #: vs. BDL group, ^#, ^##, per group).

(a)

(b)

(c)

(d)