In Vitro Antioxidant, Anti-inflammatory, and In Vivo Anticolitis Effects of Combretin A and Combretin B on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice

Table 1

Effect of Combretin A and Combretin B on protein denaturation, cyclooxygenase (COX), and 5-lipoxygenase (LOX) inhibition.

Treatment	Dose (μg/mL)	Activity			Inhibition (%)
		Protein denaturation	COX	5-LOX	Protein denaturation	COX	5-LOX
Control	-		-	-	-	-	-
Diclofenac	100	^c	-	-	74.41	-	-
	200	^c	-	-	76.72	-	-
	500	^c	-	-	81.48	-	-
	1000	^c	-	-	89.19	-	-
Ibuprofen	100	-			-	83.51	83.16
	200	-	^c	^c	-	88.79	87.41
	500	-	^c	^c	-	93.06	91.36
	1000	-	^c	^c	-	97.51	95.65
Combretin A	100	^c	^b	^b	42.49	47.58	44.25
	200	^c	^c	^c	50.92	59.58	60.87
	500	^c	^c	^c	55.41	63.06	67.64
	1000	^c	^c	^c	63.93	71.92	76.68
Combretin B	100	^c	^c	^c	59.23	61.94	62.22
	200	^c	^c	^c	69.44	71.04	69.77
	500	^c	^c	^c	77.82	80.58	81.15
	1000	^c	^c	^c	87.78	89.59	91.21

Each value represents the ;^b, ^c: significant difference compared to the control group. The percentage values were obtained using various concentrations of test compounds, and readings are presented as the mean of triplicates.