miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4

Figure 4

The miR-7-5p inhibitor blocked LPS-induced LX-2 cell proliferation and activation. LX-2 cells were treated with the NC or miR-7-5p inhibitor with or without 100 ng/ml LPS. (a) miR-7-5p levels in LX-2 cells measured by qRT-PCR. (b) OD 450 was measured for 0, 12, 24, and 48 hours later with CCK-8 reagents. (c) The levels of hydroxyproline content in LX-2 cells measured with a hydroxyproline assay kit. (d) mRNA levels of FGFR4, TGF-β1, α-SMA, and COL1A1. (e) Protein levels of FGFR4, TGF-β1, α-SMA, and COL1A1 measured by western blot. LPS: lipopolysaccharides; NC: scramble-miR control. , , and versus control; ^#, ^##, and ^### versus NC+LPS.

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