miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4

Figure 5

FGFR4 overexpression inhibited the proliferation and activation of LX-2 cells triggered by miR-7-5p. LX-2 cells were cotransfected with the NC or miR-7-5p mimic and empty vector or FGFR4 overexpression vector. (a) miR-7-5p levels in LX-2 cells were measured by qRT-PCR. (b) OD 450 was measured for 0, 12, 24, and 48 hours later with CCK-8 reagents. (c) The levels of hydroxyproline content in LX-2 cells measured with a hydroxyproline assay kit. (d) mRNA levels of FGFR4, TGF-β1, α-SMA, and COL1A1 measured by qRT-PCR. (e) Protein levels of FGFR4, TGF-β1, α-SMA, and COL1A1 measured by western blot. NC: scramble-miR control; Vector: empty vector; oeFGFR4: FGFR4 overexpression vector. , versus NC+Vector; ^##, ^### versus mimic+Vector; , versus NC+oeFGFR4.

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