miR-21 Overexpression Promotes Esophageal Squamous Cell Carcinoma Invasion and Migration by Repressing Tropomyosin 1

Figure 3

miR-21 targets and represses TPM1 in ESCC. (a) A diagram showing miR-21 binding to its predicted binding site in the TPM1 3UTR. (b) The relative luciferase activity of the TPM1-3UTR-WT group after miR-21 interference in EC109 cells. (c) The relative luciferase activity of the TPM1-3UTR-MT group after miR-21 interference in EC109 cells. (d) The miR-21 relative expression value in 8 ESCC tissues and matched adjacent normal tissues. (e) The TPM1 protein expression in 8 ESCC tissues and matched adjacent normal tissues. (f) The gray scale ratio of the TPM1/GAPDH protein expression and miRNA-21 expression. (g) The expression of TPM1 in ESCC cell lines and normal tissue. (h) The miR-21 relative expression value after miR-21 interference in EC109 and EC1 cells. (i) The TPM1 protein expression after miR-21 interference in EC109 and EC1 cells (, ).

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