lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression

Figure 4

The oncogenic ability of GAU1 is GALNT8 dependent, and overexpression of GAU1/GALNT8 axis sensitizes CRC cell lines to chemotherapy. (a) CCK-8 assay and (b) colony formation assay were used to measure proliferation in SW620 and HCT116 cells transfected with pCDH-GALNT8 or pCDH. ; . (c) CCK-8 assay and (d) colony formation assay were conducted in control siRNA (siNS) or siRNAs (siGALNT8-1 and siGALNT8-2) against GALNT8-transfected DLD1 and LoVo cells. ; . (e, f) Proliferation analysis by (e) CCK-8 assay and (f) colony formation assay in GAU1-overexpressed SW620 and HCT116 cells transfected with control siRNA (siNS) or siRNAs (siGALNT8-1 and siGALNT8-2) against GALNT8. ; . (g) Viability of pCDH-GAU1- or pCDH-transfected SW620 and siGAU1-1/2- or siNS-transfected DLD1 cells incubated with multiple concentrations of oxaliplatin (0.01-40 μM) was monitored through CCK-8 assay.

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