HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling

Figure 3

miR-185 directly targeted UCA1. (a) Target scheme of miR-185 and UCA1 3UTR. The seed sequence of the target was highlighted with red. (b) Ectopic expression of miR-185 by miRNA mimics was confirmed by q-PCR analysis in SW480 cells. (c) UCA1 expression was inhibited by miR-185 confirmed by q-PCR analysis in SW480 cells. (d) The EMT maker expression level was confirmed by q-PCR analysis in SW480 with UCA1 miR-185 mimic transfection. (e) The EMT maker protein level was confirmed by Western blot in SW480 with UCA1 miR-185 mimic transfection. (f) Q-PCR analysis of miR-185 expression in four different CRC cell lines. The UCA1 expressions were compared to HCnEpC cells. (g) The target of miR-185 and UCA1 was confirmed by luciferase assay. (h) Q-PCR analysis of miR-185 expression after ectopically expressed UCA1 or silenced UCA1. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)