Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6

Figure 2

Knockdown of SNHG7 reverses ATB resistance in CRC cells. (a) qRT-PCR revealing the upregulation of SNHG7 in oe-SNHG7 cell lines (HCT116/ATB-oeSNHG7 and LOVO/ATB-oeSNHG7 cell lines). (b) Survival rate of the SNHG7-overexpressing HCT116/ATB and LOVO/ATB cell lines treated with 0, 2, 5, 10, and 20 μM ATB. (c) CCK8 assay examining the cell viability of oeSNHG7 cell lines. (d) qRT-PCR measurement to check the expression of SNHG7 in siSNHG7 cell lines (HCT116/ATB-siSNHG7 and LOVO/ATB-siSNHG7 knockdown cell lines). (e) Survival rate of HCT116/ATB and LOVO/ATB cell lines with SNHG7 knockdown that are treated with 0, 2, 5, 10, and 20 μM ATB. (f) CCK8 assay showing the decreased cell viability of siSNHG7 cell lines. (g) Clone-forming ability of HCT116/ATB and LOVO/ATB cell lines with SNHG7 knockdown. (h) Flow cytometric analysis detecting the apoptosis level of siSNHG7 cell lines and the control. Down-regulation of SNHG7 in HCT116/ATB and LOVO/ATB cell lines increases cell apoptosis. (i) Western blot determining the protein level of caspase-3 and Bcl-2. The expression level of caspase-3 was increased, whereas Bcl-2 was decreased in siSNHG7 cell lines. The statistical differences were evaluated by Student’s t-test (*, **, and ***).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)