Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6

Figure 3

MiR-181a-5p is directly regulated by SNHG7. (a) Potential binding site of miR-181a-5p in SNHG7 predicted by StartBase3 and confirmed by Luciferase reporter assay in HCT116/ATB and LOVO/ATB cell lines. Overexpression of miR-181a-5p could inhibit Luciferase activity, but the inhibition was abolished when the potential site was mutated. (b) RNA pull-down assay showing that SNHG7 fragments were enriched effectively using the miR-181a-5p probe in HCT116/ATB and LOVO/ATB cell lines. (c) RIP-qRT-PCR showing SNHG7 and miR-181a-5p fragments were enriched by Ago2 in HCT116/ATB and LOVO/ATB cell lines. (d) qRT-PCR analysis of the expression of miR-181a-5p in 50 pairs of CRCs and the adjacent tissues. (e) StartBase3 analysis of the expression of miR-181a-5p in the data from TCGA. (f) Spearman correlation analysis revealing a significant negative correlation between SNHG7 and miR-181a-5p in 50 CRC cases. The statistical differences were evaluated by Student’s t-test (*, **, and ***).

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