Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6

Figure 5

SNHG7 knockdown improves the ATB-sensitivity of CRC by regulating miR-181a-5p/GATA6. (a) Survival rate of HCT116/ATB and LOVO/ATB cell lines treated with 0, 2, 5, 10, and 20 μM ATB. SNHG7 knockdown decreased the survival rate, which was partially recovered by overexpressing GATA6 or miR-181a-5p inhibitor. (b) Cell viability of siSNHG7 cell lines. The decreased cell viability was partially recovered by overexpressing GATA6 or miR-181a-5p inhibitor. (c) Flow cytometric analysis of the level of cell apoptosis. Down-regulation of SNHG7 in HCT116/ATB and LOVO/ATB cell lines increased cell apoptosis, which was partially reversed by overexpressing GATA6 or miR-181a-5p inhibitor. (d) Western blot to determine the levels of caspase-3 and Bcl-2, apoptosis-related proteins. Caspase-3 expression was increased, but Bcl-2 expression was decreased in siSNHG7 cell lines, which was partially reversed by overexpressing GATA6 or miR-181a-5p inhibitor. The statistical comparison was done by Student’s t-test (*, **, and ***).

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