Functional Characterization of Novel Lunatic Fringe Variants in Spondylocostal Dysostosis Type-III with Scoliosis

Figure 5

The p.G256S and p.R174H LFNG substitutions localize to the Golgi. (a) WT, p.F188L (b), p.G256S (c), and p.R174H (d) LFNG-HA variants transiently transfected NIH3T3 cells. Cells were incubated with Hoechst, rabbit anti-HA, and mouse anti-GM130 primary antibodies and then again with fluorescent secondary antibodies. The fluorescent signal was artificially altered to be color-blind friendly. Note the absence of signal colocalization in (b). Images are representative samples of three independent experiments (). Scale bar, 30 μm.

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