A Novel Constitutively Active , p.(R33P) Variant in RAB11A Associated with Intellectual Disability Promotes Neuritogenesis and Affects Oligodendroglial Arborization

Figure 3

Effect of RAB11A-R33P expression on neuronal morphology. (a–e) Representative images of primary cultured hippocampal neurons labeled with anti-GFP (green) and antiphosphorylated neurofilament (SMI-312, magenta). Hippocampal neurons infected with lentiviral EGFP alone (a) or EGFP-RAB11A WT (b), -RAB11A-R33P (c), -Rab11A-S25N (d), and -Rab11A-Q70L (e) at 0 DIV were analyzed at 4 DIV. Cell bodies and axon terminals are indicated by arrows and arrowheads, respectively. (f) A graph showing quantified axonal lengths in each group. Values () are indicated as dots and the in each group is presented as bars. . (g–k) Representative reconstructed 3D images of primary cultured hippocampal neurons infected with lentiviral vectors as in (a–e) and labeled with anti-GFP and anti-MAP2. Cell bodies stained with anti-GFP alone are blue-colored, and dendrites stained with anti-MAP2 are gray-colored in all images. Graphs showing quantified total lengths of dendrites (l) and numbers of branching points of dendrites (m) in each group. Values () are indicated as dots and the in each group is shown as bars. , , .

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