A De Novo Noncoding RARB Variant Associated with Complex Microphthalmia Alters a Putative Regulatory Element

Figure 5

Overexpression of RARB leads to increased cell proliferation in human lens epithelial cells. (a) Relative changes in RARB mRNA expression were assessed via RT-qPCR. RNA was extracted from human lens epithelial cells transfected with either human RARB expression vector or empty pcDNA3.1/V5-His TOPO TA expression vector. (b) Total cell number, (c) doubling rate, and (d) viability were calculated using the Trypan blue exclusion method. Data were derived from three independent replicates, each performed in duplicate. Unpaired -tests were used to determine the statistical significance of any differences between transfected and control transfected samples. , , and . ns = nonsignificant. Error bars represent standard deviation.

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